Gene transfer: transformation/electroporation
| Autor: | Cadoret, Frederic, Soscia, Chantal, Voulhoux, Romé |
|---|---|
| Přispěvatelé: | Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2014, 1149, pp.11--5. ⟨10.1007/978-1-4939-0473-0_2⟩ Methods in Molecular Biology, 2014, 1149, pp.11--5. ⟨10.1007/978-1-4939-0473-0_2⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-4939-0473-0_2⟩ |
| Popis: | International audience; Since Pseudomonas aeruginosa is a non-naturally competent bacterium, various methods have been developed to transfer exogenous DNA. Alternatively to transduction and conjugation, electroporation can also be used to transfer exogenous DNA molecules into Pseudomonas. Electroporation uses an electric field which generates pores in bacterial membranes allowing the entry of the exogenous DNA molecule. In contrast to conjugation which is restricted to the transfer of DNA from one bacterial cell to another, electroporation can be used to transfer all types of DNA resuspended in water. |
| Databáze: | OpenAIRE |
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