Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction

Autor:	Bonants, P., Hagenaar-de Weerdt, M., van Gent-Pelzer, M., Lacourt, I., Cooke, D., Duncan, J.
Jazyk:	angličtina
Rok vydání:	1997
Předmět:	Phytophthora internal transcribed spacer nested PCR ribosomal DNA PCR-ELISA Instituut voor Plantenziektenkundig Onderzoek food and beverages Research Institute for Plant Protection ITS
Zdroj:	European Journal of Plant Pathology 103 (1997) European Journal of Plant Pathology, 103, 345-355
ISSN:	0929-1873
Popis:	Phytophthora fragariae Hickman, which causes strawberry red stele and raspberry root rot, is a quarantine organism for which specific and sensitive detection methods are required to test the health of planting material. Sequences of the internal transcribed spacer regions of the ribosomal gene repeat (rDNA) were used to develop primers for P. fragariae in a nested Polymerase Chain Reaction (PCR). The fungus was readily detected in infected but symptomless roots by nested, but not single-round, PCR. It was also detected in infested water samples obtained from the Dutch General Inspection Service by nested PCR. Detection of PCR products was at least 10-fold more sensitive by PCR-ELISA than by conventional visualisation on agarose gels.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::9d03be2dfbb781f3b53db209ba24150b https://research.wur.nl/en/publications/detection-and-identification-of-phytophthora-fragariae-hickman-by Zobrazit plný text záznamu Full text from SpringerLink