COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE
| Autor: | N. Abbasi, S. Khaghani, A. Sharif-Tabrizi, B. Farzami, S. Vardasbi, M. Bahar, M. Ansari, R. Khorramizadeh S. Gerayesh-Nejad |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Acta Medica Iranica, Vol 45, Iss 4 (2007) Acta Medica Iranica, Vol 45, Iss 4, Pp 282-284 (2007) |
| ISSN: | 1735-9694 0044-6025 |
| Popis: | To determine the importance of Q10H2 as an antioxidant in cancer, we measured the activity of lipoamide dehydrogenase (Q10H2 recycling enzyme) in HL-60 and normal lymphocyte. The cultured cells of HL-60 and human normal lymphocytes were assayed in cell lysate of given number of both HL-60 and normal lymphocyte. The activity of lipoamide dehydrogenase and the protein concentration were determined by spectrometric methods. The activity of LAD was found to be 0.216µmol/min/µg protein in HL-60 cells and 0.0415µmol/min/µg protein in normal lymphocytes. Although the average protein concentration ratio in HL-60 cells to normal lymphocyte was found to be %124, the average ratio of enzyme activity in HL-60 to normal lymphocyte was %526. These results are indicative of independence of enzyme increase in HL-60 cells of increase of protein synthesis. |
| Databáze: | OpenAIRE |
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