Changes in gene expression following exposure of bovine endometrial epithelial cells to E Coli LPS
| Autor: | Guo, Y., Van Schaik, T., Chanrot, M., Niazi, A., Jhamat, N., Valarcher, Jean Francois, Charpigny, Gilles, Bongcam-Rudloff, E., Andersson, G., Humblot, Patrice |
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| Přispěvatelé: | Department of Clinical Sciences, Division of Reproduction, Faculty of Veterinary Medicine and Animal Science, Department of Animal Bredding and Genetics, Swedish University of Agricultural Sciences (SLU), Uppsala University, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), European Cooperation in Science and Technology (COST). BEL. |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Conference 2015-Epigenetics and Periconception Environment Conference 2015-Epigenetics and Periconception Environment, European Cooperation in Science and Technology (COST). BEL., Oct 2015, Hersonissos, Greece |
| Popis: | International audience; Lipopolysaccharide (LPS) is a component of outer membrane of Gram-negative bacteria involved in post-partum metritis and endometritis in cattle. LPS causes inflammation of the endometrium. Increased cell proliferation is part of the inflammatory process and has been reported in human epithelial and immune cells and from bovine Endometrial epithelial Cells (bEEC). In this study we investigated possible changes in gene expression in relation with the proliferative response of bEEC after challenge with E. coli-LPS. Following In vitro culture, bEEC from 3 cows were exposed to 0, 2, and 8 μg/ml LPS for 24 hrs. For each time point and LPS dosage, attached cells were counted then frozen. The variation of cells number over time was analyzed by ANOVA (SAS 9.1, proc GLM). RNA was extracted by using the All prep DNA/RNA Universal kit (Qiagen). Samples were analyzed by RNA sequencing performed in SciLife Laboratory in Uppsala and differentially expressed genes (DEG) searched by using Ensemble genes as reference. A significant increase in cell number was observed for cells treated with 8 μg/ml LPS (P≤ 0.001) whereas proliferation with 2μg/ml was more moderate. Major changes in gene expression were observed whatever the dosage used. 2035 DEG were found (Benjamini-Hochberg adjusted P value |
| Databáze: | OpenAIRE |
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