Itraq-based proteomics analysis of citrate gold nanoparticle exposure effects in the marine clam Ruditapes phlippinarum

Autor: Volland, Moritz, Torreblanca, Amparo, Blasco, Julián, Hampel, Miriam
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: Trabajo presentado en el 10th Iberian Congress on Environmental Contamination and Toxicology y el 7th Iberoamerican Congress on Environmental Contamination and Toxicology (CICTA 2015), celebrados en Vila Real (Portugal) del 14 al 17 de julio de 2015.
Engineered Gold nanoparticles (AuNPs) are introduced into a growing number of commercial products. Notwithstanding their promising applications, the increased likelihood of their release into environmental compartments and the resulting exposure on the species within needs to be met by an increased understanding of their behaviour and effects. Up to date limited information is available on the ecotoxicological risk for non-target organisms, with the majority of research focusing on magnified expectable environmental concentrations. In our laboratory-based study the bivalve Ruditapes philippinarum was chosen as a model to evaluate differential protein expression of citrate capped AuNPs (21.5 ± 2.9 nm) at an environmental relevant concentration (0.75 μg L-1). We used a 2nd-generation (iTRAQ-8plex) proteomic approach to identify and quantify differently expressed proteins in the digestive gland after 1 and 7 days of exposure in comparison to control organisms.The iTRAQ results demonstrate that the digestive gland is affected by environmentally relevant concentrations of citrate capped gold nanoparticles. Over 2500 proteins involved in a wide range of metabolic pathways and/or physiological processes were identified. Altered proteins may be useful as biomarkers of environmental nanoparticle pollution and provide insight about the mode of action of engineered gold nanoparticles.
Databáze: OpenAIRE
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