What are the mechanisms of gastrointestinal lipases adsorption onto heterogenous biomimetic vegetal membranes?
| Autor: | Kergomard, Jeanne, Carrière, F, Lambeau, G, Paboeuf, Gilles, Barouh, Nathalie, Villeneuve, Pierre, Bourlieu-Lacanal, Claire, Vié, V. |
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| Přispěvatelé: | Kergomard, Jeanne, Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Démarche intégrée pour l'obtention d'aliments de qualité (UMR QualiSud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Avignon Université (AU)-Université de La Réunion (UR)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
[PHYS]Physics [physics]
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition [CHIM.ANAL] Chemical Sciences/Analytical chemistry [CHIM.ANAL]Chemical Sciences/Analytical chemistry [PHYS.COND]Physics [physics]/Condensed Matter [cond-mat] [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition [PHYS.COND] Physics [physics]/Condensed Matter [cond-mat] [PHYS] Physics [physics] |
| Zdroj: | 7th International Conference on Food Digestion ICFD 2022 7th International Conference on Food Digestion ICFD 2022, May 2022, Cork, Ireland., pp.95 |
| Popis: | International audience; Gastrointestinal lipases are crucial for lipid hydrolysis and must get adsorbed onto the substrate interface prior lipolysis. Such adsorption had not been deeply investigated on biomimetic vegetal membranes. Our objective was thus to characterize such adsorption and lipolysis using complementary biophysical tools (tensiometry, ellipsometry, atomic force microscopy) and in vitro digestion.Heterogenous monolayers based on galactolipids, phospholipids, and phytosterols were used. Four lipases were studied: i) gastric lipase (GL), ii) pancreatic lipase 2 (PLRP2), (iii) pancreatic triacylglycerol lipase and its cofactor, colipase (PTL/coPTL), and (iv) pancreatic secreted phospholipase A2 (sPLA2-IB).A strong surfactant property of GL and its preferential adsorption onto expanded lipid phase and at the phase boundary were observed, in line with previous results on milk fat globule membrane. With PLRP2, changes in surface pressure indicated a lipolytic activity. Such variation was not observed upon PTL/coPTL adsorption, and may be related to the absence of activity of PTL on polar lipids. The injection of sPLA2-IB did not indicate a clear trend of lipolysis on the lipid film but changed the morphology of condensed domains.This study is a step forward to understand the interactions of gastrointestinal lipases with plant lipid membranes, an overlooked aspect of lipid digestion |
| Databáze: | OpenAIRE |
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