Identification of different Escherichia coli pathotypes in north and north-west provinces of Iran
| Autor: | Miri, S. T., Dashti, A., Saied Mostaan, Kazemi, F., Bouzari, S. |
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| Přispěvatelé: | Molecular Biology Department [Tehran, Iran], Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), This work was financially supported by Pasteur Institute of Iran and Ministry of Health. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Diarrhea
fluids and secretions [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases lcsh:QR1-502 Escherichia coli bacteria Pathotypes biochemical phenomena metabolism and nutrition Iran bacterial infections and mycoses [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology lcsh:Microbiology |
| Zdroj: | Iranian Journal of Microbiology Iranian Journal of Microbiology, Tehran University of Medical Sciences, 2017, 9 (1), pp.33-37 Scopus-Elsevier Iranian Journal of Microbiology, Vol 9, Iss 1 (2017) |
| ISSN: | 2008-3289 2008-4447 |
| Popis: | International audience; Background and Objectives: Diarrhea is one of the most prevalent diseases in the world, specially in developing countries. One of the most important causative agents of bacterial diarrhea is diarrheagenic Escherichia coli (DEC) which causes gastroenteritis and this group involving enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterohemoragic E. coli (EHEC), enteroinvasive E. coli (EIEC), diffusely adherence E. coli (DAEC). The aim of this study was to identify different E. coli pathotypes in north and north-west of Iran, among the clinical isolates.Materials and Methods: In this study for identification of E. coli, 170 fecal samples were cultured on MacConkey agar and identified by biochemical tests. Samples with E. coli characteristics were selected (145 samples) and their genomes were purified by phenol-chloroform method. After extraction of genomes, lt and sta genes identified by PCR for ETEC, eae gene for atypical and eae and bfp for typical EPEC, AA region for EAEC, stx1 and stx2 and eae genes for EHEC (stx1 or stx2 or both for STEC) and invE for EIEC.Results: Finally 10 samples identified as ETEC (%5.88), 18 (%10.58) EPEC, 6 (%3.52) EHEC and 12 (7.05%) samples were STEC. None of the samples were positive for EAEC and EIEC.Conclusion: The results obtained in this study showed that ETEC, EPEC, EHEC and STEC are prevalent bacterial agents in north and north-west of Iran. Complementary studies to identify these pathotypes in other seasons can help to adopt necessary policies against outbreaks in Iran. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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