| Popis: |
Bu tezde, gilaburu (Viburnum opulus L.) meyve suyunun organik asit ve fenolik bileşikleri Zıt-Faz Yüksek Basınç Sıvı Kromatografisi ile tayin edilmiştir. Organik asitlerden L-malik asit, okzalik asit, tartarik asit, L-askorbik asit belirlenmiştir. Gilaburu meyve suyundaki organik asitlerin miktarları; L-malik asit 9.422+0.032 g/L, okzalik asit 0.573+0.070 g/L, tartarik asit 0.095+0.010 g/L, L-askorbik asit 0.736+0.030 g/L dir. Organik asitlerin bu yöntemle geri kazanımları %88.4-%128.3 arasında bulunmuştur. Gilaburu suyunda fenolik bileşiklerden klorogenik asit, kafeik asit, p-kumarik asit, p-hidroksibenzoik asit ve mirisetin bileşikleri belirlenmiştir. Fenolik bileşiklerin miktarlarının; klorogenik asit 798.81+6.49 mg/L, kafeik asit 26.22+0.05 mg/L, p-hidroksibenzoik asit 92.10+0.059 mg/L, p-kumarik asit 3.38+0.04 mg/L, mirisetin 35.97+0.64 mg/L olduğu belirlenmiştir. Fenolik bileşiklerin bu yöntemle geri kazanımları %61.3-%89.4 arasında bulunmuştur. Anahtar kelimeler: Viburnum opulus L.,gilaburu, fenolik bileşikler, organik asit, klorogenik asit, L-malik asit, HPLC In this thesis, organic acids and phenolic compounds of gilaburu (Viburnum opulus L.) fruit juice were determined by Reversed Phase High Pressure Liquid Chromatography (RP- HPLC). Organic acids such as L-malic, tartaric, oxalic and L- ascorbic acids were individually detected. 9.422+0.032 g/L of L- malic acid, 0.573+0.070 g/L of oxalic acid, 0.095+0.010 g/L of tartaric acid and 0.736+0.030 g/L of L-ascorbic acid were quantified in gilaburu fruit juice. The recoveries of organic acids were found within the range of 88.4-128.3 % by this method. Phenolic compounds identified in gilaburu fruit juice were chlorogenic acid, caffeic acid, p-coumaric acid, p-hydroxybenzoic acid and myricetin. 798.81+6.49 mg/L of chlorogenic acid, 26.22+0.05 mg/L of caffeic acid, 92.10+0.059 mg/L of p- hydroxybenzoic acid, 3.38+0.04 mg/L of p- coumaric acid, 35.97+0.64 mg/L of myricetin were quantified in gilaburu fruit juice. The recoveries of phenolic compounds were found within the range of 61.3-89.4 % by this method. Keywords: Viburnum opulus L., gilaburu, phenolic compounds, organic acids, chlorogenic acid, L-malic acid, HPLC 84 |
