The validation of analytical methods as an evaluation tool of research results reliability
| Autor: | Ollivier, Elodie, Jaulin, Anne, GRONDIN, Virginie, Trouve, Amelie, Cheviron, Nathalie |
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| Přispěvatelé: | Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
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| Zdroj: | SETAC Europe 26th Annual Meeting SETAC Europe 26th Annual Meeting, May 2016, Nantes, France. 2016 |
| Popis: | National audience; Biochem-Env is a platform for analytical biochemistry created in 2012 by INRA with the support of the program “Investissements d’avenir” ANAEE-France. The mission of Biochem-Env is to provide facilities for the biochemical characterization of natural environments (enzyme activities in soils and sediments) and associated macrofauna (enzyme activities, phospholipid fatty acids, terrestrial and benthic macrofauna biomarkers), as well as lipidic biomarkers (PLFAs). Partner in research projects, the platform must produce traceable analytical data with high level of confidence. INRA’s quality policy requests the validation of analytical methods when laboratories use non-standardized ones and methods used outside the scope of application of the dedicated norm. For intra-laboratory validation of quantitative analytical methods, the INRA’s Quality Guidelines for research and experimental units (2013) recommends “the accuracy profile”method according to the NF V03-110: 2010 standard. This approach has the advantage of providing an overall statistical method combining trueness and precision by simple and graphic interpretation and allowing a rapid decision. Furthermore, it enables the determination of quantification limits, the scope of the application of the method and the estimation of uncertainty associated with the results. The process of analytical method validation allows establishing fitness for purpose by the interpretation of criteria in relation to the scientific objectives. The objective of this study was to evaluate and validate the performance of such an internally developed method applied to a set of species of terrestrial and benthic macrofauna. We expressed in particular interest in protein determination method by the bicinchoninic acid, using a commercial kit. The method was applied to 7 earthworm species, one of beetle, spider and gammarids. The validation method helped us to assess the matrix effect and to improve the steps for sample preparation and analysis. It also pointed out the importance of an experimented analyst to apply the analytical method. |
| Databáze: | OpenAIRE |
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