Leaf patch clamp pressure probe measurements on olive leaves in a nearly turgorless state

Autor: Ehrenberger, W., Rüger, S., Rodríguez Domínguez, Celia M., Díaz-Espejo, Antonio, Fernández Luque, José Enrique, Moreno Onorato, F. J., Zimmermann, D., Sukhorukov, V. L., Zimmermann, U.
Jazyk: angličtina
Rok vydání: 2012
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1438-8677
1435-8603
Popis: The non-invasive leaf patch clamp pressure (LPCP) probe measures the attenuated pressure of a leaf patch, P p, in response to an externally applied magnetic force. P p is inversely coupled with leaf turgor pressure, P c, i.e. at high P c values the P p values are small and at low P c values the P p values are high. This relationship between P c and P p could also be verified for 2-m tall olive trees under laboratory conditions using the cell turgor pressure probe. When the laboratory plants were subjected to severe water stress (P c dropped below ca. 50kPa), P p curves show reverse diurnal changes, i.e. during the light regime (high transpiration) a minimum P p value, and during darkness a peak P p value is recorded. This reversal of the P p curves was completely reversible. Upon watering, the original diurnal P p changes were re-established within 2-3days. Olive trees in the field showed a similar turnover of the shape of the P p curves upon drought, despite pronounced fluctuations in microclimate. The reversal of the P p curves is most likely due to accumulation of air in the leaves. This assumption was supported with cross-sections through leaves subjected to prolonged drought. In contrast to well-watered leaves, microscopic inspection of leaves exhibiting inverse diurnal P p curves revealed large air-filled areas in parenchyma tissue. Significantly larger amounts of air could also be extracted from water-stressed leaves than from well-watered leaves using the cell turgor pressure probe. Furthermore, theoretical analysis of the experimental P p curves shows that the propagation of pressure through the nearly turgorless leaf must be exclusively dictated by air. Equations are derived that provide valuable information about the water status of olive leaves close to zero P c.
This work was supported by a grant from the BMWi and the ESF (no. FKZ 03EGSBY137) to S.R. and U.Z. and a grant of the Europaïscher Fonds für regionale Entwicklung (EFRE 80145650; State Brandenburg) to U.Z. The fieldwork was funded by the Spanish Ministry of Science and Innovation (research project AGL2009-11310 ⁄AGR).
Databáze: OpenAIRE
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