| Autor: |
Bolaman, Z., Akalin, N., Koseoglu, M.H., Demir, S., Kadikoylu, G., Atalay, H., Aslan, D. |
| Jazyk: |
angličtina |
| Rok vydání: |
2003 |
| Předmět: |
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| Popis: |
To investigate the effect of amifostine (AMI, WR-2721) against lipid peroxidation caused by adriamycin in rat heart tissue, male Wistar rats, weighing 190-220 g, were divided into three groups and treated as follows: 1) vehicle (saline) control (n = 6); 2) Adriamycin (10 mg/kg, intraperitoneally) (n = 6); 3) Adriamycin plus amifostine (200 mg/kg) (n = 6). Amifostine was applicated to rats 30 minutes previously from Adriamycin application. Rats were sacrificed on third day after drug application and hearts were removed. The hearts were washed with cold saline solution and were stored at -20°C until analysed. The tissues were thawed and homogenised with an Ultra Turrax. Lipid peroxidation was assayed as the malondialdehyde (MDA) levels reacting with thiobarbituric acid, according to the method of Ohkawa. Tissue MDA levels were significantly increased by adriamycin (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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