| Popis: |
Poly(A)-enriched $RNA$ from either liver or oviduct of estradiol -17/beta treated immature chicks supported $[3H]$ leucine incorporation into immunoprecipitable riboflavin-carrier protein in a dose-dependent manner when translated in the rabbit reticulocyte lysate system. Primary translation product of riboflavin-carrier protein had a molecular weight of 38,000, which on incubation with a stripped hepatic microsomal preparation was processed to a product with a size comparable to native riboflavin-carrier protein. Poly(A)-enriched $RNA$ from both the liver and the oviduct of estrogen-treated birds stimulated $[^3H]$ -leucine incorporation into riboflavin-carrier protein, and this was 2-3-fold higher during secondary stimulation vis-a-vis primary stimulation with the steroid. Poly A-enriched RNA from the liver of progesterone -treated birds during secondary stimulation did not support riboflavin-carrier protein synthesis. In contrast, poly A-enriched $RNA$ from the oviduct of the birds treated with progesterone during secondary (but not primary) stimulation did exhibit riboflavin-carrier protein-$mRNA$ activity which was comparable to that stimulated by estradiol- 17\beta. |
