An Asian commercial strain of Agrocybe chaxingu and a European wild strain of Agrocybe cylindracea exhibiting morphological difference and high genetic divergence are interfertile

Autor: Philippe Callac, Jacques Guinberteau, Cyril Férandon, Gérard Barroso
Přispěvatelé: Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Université de Bordeaux Ségalen [Bordeaux 2], Institut National de Recherche Agronomique (INRA). UR Unité de recherche Mycologie et Sécurité des Aliments (1264).
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: 7. International Conference on Mushroom Biology and Mushroom Products
7. International Conference on Mushroom Biology and Mushroom Products, Institut National de Recherche Agronomique (INRA). UR Unité de recherche Mycologie et Sécurité des Aliments (1264)., Oct 2011, Arcachon, France
HAL
Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7) 2011. 2011; 7. International Conference on Mushroom Biology and Mushroom Products, Arcachon, FRA, 2011-10-04-2011-10-07, 113-122
Popis: Three molecular markers were compared between a cultivated strain of Agrocybe chaxingu from South-Eastern Asia (SM960903) and a French wild strain of Agrocybe cylindracea (SM47): (i) the nuclear ribosomal unit, (ii) the mitochondrial SSU-rDNA and (iii) the mitochondrial cob gene. All markers reveal extensive variations: the ribosomal units possess up to 67 polymorphic sites (89% of nt identity), the variable domains of the mitochondrial SSU-rDNA differ by large indel sequences and the cob gene molecular organization is characterized by non-orthologous group I introns. In fruiting tests, sporophores of the two strains were macroscopically distinguishable, especially by a thicker and stronger annulus in A. chaxingu. In inter-stock mating tests, hyphal clamp connections were detected in mycelia of the junction lines for all the confrontations. The dikaryotic status of the presumed hybrids was confirmed by the presence of both A. chaxingu and A. cylindracea nuclear ribosomal units. Dikaryotization was also observed in distal regions from the junction lines, indicating that nuclei migration occurred in these matings. Four selected hybrids containing each of the four A. cylindracea mating type genotypes were put in fruiting conditions. These hybrid sporophores were molecularly verified and shown to be fertile based on the presence of mature basidiospores. Despite extensive variations at the molecular and phenotypic levels, the complete inter-fertility observed between the Asian A. chaxingu and the European A . cylindracea strains strongly argues for con-specificity of both taxa. A. chaxingu would be a variety of the A. cylindracea species which was firstly described.
Databáze: OpenAIRE
Externí odkaz: