| Autor: |
Ingrid Zegers, Luigi Carotenuto, Christine Evrard, Juan Ma Garcia-Ruiz, Philippe De Gieter, Luis Gonzales-Ramires, Eric Istasse, Jean-Claude Legros, Christopher Minetti, Fermin Otalora, Patrick Queeckers, Cedric Schockaert, Cécile Van De Weerdt, Ronnie Willaert, Lode Wyns, Catherine Yourassowsky, Frank Dubois |
| Přispěvatelé: |
Structural Biology Brussels, Ultrastructure |
| Předmět: |
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| Zdroj: |
Vrije Universiteit Brussel |
| Popis: |
The crystallisation by counterdiffusion is a very efficient technique for obtaining high-quality protein crystals. A prerequisite for the use of counterdiffusion techniques is that mass transport must be controlled by diffusion alone. Sedimentation and convection can be avoided by either working in gelled systems, working in systems of small dimensions, or in the absence of gravity. We present the results from experiments performed on the ISS using the Protein Microscope for the International Space Station (PromISS), using digital holography to visualise crystal growth processes. We extensively characterised three model proteins for these experiments (cablys3*lysozyme, triose phosphate isomerase, and parvalbumin) and used these to assess the ISS as an environment for crystallisation by counterdiffusion. The possibility to visualise growth and movement of crystals in different types of experiments (capillary counterdiffusion and batch-type) is important, as movement of crystals is clearly not negligible. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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