Impact de la pasteurisation sur la digestion gastro-intestinale du lait maternel : digestion dynamique in vitro aux stades nouveau-nés prématuré et à terme

Autor: DE OLIVEIRA, Samira, Deglaire, Amélie, Ménard, Olivia, Briard-Bion, Valérie, Carriere, Frederic, Dirson, Emelyne, Jardin, Julien, Dupont, Didier, Bourlieu-Lacanal, Claire
Přispěvatelé: Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Enzymologie interfaciale et de physiologie de la lipolyse (EIPL), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Lactarium - Unité Nutrition et Diététique Infantile, Centre Hospitalier Universitaire [Rennes], Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: 3.International Conference on Food Structures, Digestion and Health
3.International Conference on Food Structures, Digestion and Health, Oct 2015, Wellington, New Zealand. 2015
Popis: Mother’s own milk is the ideal infant food for optimal nutrition and growth. When not available, pasteurized donor milk can be administered. Pasteurization has structural and biochemical consequences on term milk digestion1,2. Whether similar results would be observed for preterm milk remains unknown and was thus the aim of our study. An in vitro dynamic system was used to simulate digestion. Preterm human milk (n=5donors) was digested using preterm infant digestive parameters while for term milk (n=5 donors), term infant digestive parameters were employed3,4. Each type of milk was digested as raw or pasteurized (62.5°C, 30 min) in triplicate. Digesta were sampled regularly in the gastric compartment (over 90 or 120 min for preterm or term milk, respectively) and over 180 min in the intestinal compartment. Structural changes were evaluated by confocal microscopy and laser light scattering. Proteolysis was followed by SDS-PAGE submitted to tensitometry and by quantitative peptide analysis using tandem mass spectrometry coupled to liquidchromatography. Lipolysis was evaluated by gas chromatography and thin layer chromatography fitted to flame ionisation detector.Lipolysis degree was in overall significantly lower in pasteurized than in raw milk inboth preterm and term conditions, but the extent of the difference was lower for the preterm than for the term condition. Gastric lipolysis was somewhat lower in the preterm than in the term milk. Due to a higher pre-lipolysis degree in raw term milk, the pH of this milk was slightly lower and thus, as specified in our model, it impacted the gastric pH decrease, which was slightly lower than that for the other milks. The impact of pasteurization on gastric proteolysis differed according to the protein structure with β-casein being hydrolysedsignificantly slower and lactoferrin being hydrolysed significantly faster in term pasteurized milk compared to term raw milk. This was not statistically significant in preterm milk. Similarly, the peptidome differed between raw and pasteurized milk to a lesser extent in the preterm than in the term condition. In both preterm and term conditions, pasteurization impacted the protein susceptibility to aggregation during digestion. These results suggest that pasteurization may have a lower impact on digestion of preterm milk compared to term milk. The greater pre-lipolysis in raw term milk slightly impacted the gastric acidification, which may also have impacted the extent of digestive hydrolysis. Such results have to be confirmed by in vivo studies.
Databáze: OpenAIRE
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