USE OF CONJUGATES WITH LINKERS CLEAVABLE BY PHOTODISSOCIATION OR FRAGMENTATION FOR MASS SPECTROMETRY ANALYSIS OF TISSUE SECTIONS
| Autor: | Lemaire, R., Fournier, I., Salzet, M., Deschamps, M., Tabet, Jc., Proess, G., Rudloff, I., Lemaitre, M. |
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| Přispěvatelé: | Neuroimmunologie des annélides (NA), Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), UROGENTEC, Eurogentec S.A. [Seraing, Belgique], Synthèse, Structure et Fonction de Molécules Bioactives (SSFMB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ACI, CNRS DPI, MENRT |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MALDI imaging
diagnosis [CHIM.ANAL]Chemical Sciences/Analytical chemistry specific imaging mass sepctrometry [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.IB]Life Sciences [q-bio]/Bioengineering Tag mass [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | United States, Patent n° : WO2006IB02309 20060607. 2007, pp.65 |
| Popis: | The invention concerns a method for determining at least one target molecule map in a tissue section, using at least one (A-X)n-B conjugate, wherein A is a tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, n is an integer of at least l,and B is a binding molecule that binds specifically to said target molecule. When using MALDI mass spectrometry, said linker molecule X may be cleaved by photodissociation during sample laser irradiation if photocleavable at the wavelength of said MALDI laser. Alternatively, when using UV- MALDI, IR-MALDI, SIMS or DESI mass spectrometry, said linker molecule X may be cleaved by fragmentation during sample desorption/ionization. |
| Databáze: | OpenAIRE |
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