Fluorescence polarization immunoassay for rapid detection of T-2 and HT-2 toxins in cereals and cereal-based products

Autor: Anna Chiara R. Porricelli, Vincenzo Lippolis, Stefania Valenzano, Marina Cortese, Michele Suman, Sandro Zanardi, Michelangelo Pascale
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: 11th Conference RME 2016-Feed Food Water Analysis Human Animal Diagnostics, Amsterdam, 7/11/2016-9/11/2016
info:cnr-pdr/source/autori:Anna Chiara R. Porricelli, Vincenzo Lippolis, Stefania Valenzano, Marina Cortese, Michele Suman, Sandro Zanardi, Michelangelo Pascale/congresso_nome:11th Conference RME 2016-Feed Food Water Analysis Human Animal Diagnostics/congresso_luogo:Amsterdam/congresso_data:7%2F11%2F2016-9%2F11%2F2016/anno:2016/pagina_da:/pagina_a:/intervallo_pagine
2nd IMEKOFOODS Promoting Objective and Measurable Food Quality & Safety, Benevento, 2/10/2016-5/10/2016
info:cnr-pdr/source/autori:Anna Chiara R. Porricelli, Vincenzo Lippolis, Stefania Valenzano, Marina Cortese, Michele Suman, Sandro Zanardi, Michelangelo Pascale/congresso_nome:2nd IMEKOFOODS Promoting Objective and Measurable Food Quality & Safety/congresso_luogo:Benevento/congresso_data:2%2F10%2F2016-5%2F10%2F2016/anno:2016/pagina_da:/pagina_a:/intervallo_pagine
ISSN: 2481-2668
Popis: T-2 toxin (T-2) and HT-2 toxin (HT-2) are type A trichothecene mycotoxins produced by several Fusarium species, mainly Fusarium sporotrichioides, Fusarium langsethiae and Fusarium poae. Generally, these Fusarium species can grow on cereals and produce T-2 and HT-2 under moist cool conditions already prior to harvesting [1]. Among cereals, oats, wheat, rye and derived products are key dietary sources of T-2 and HT-2 exposure [1]. In order to protect consumer health from the risk of exposure to these toxins, the development of rapid, sensitive and reliable methods for their determination in cereals and cereal-based products is needed. Fluorescence polarization (FP) immunoassay is a homogeneous technique that is getting attention as a screening tool in food safety control due to its simplicity, rapidity, cheapness and reliability. A rapid, sensitive and reliable FP immunoassay has been recently reported for the determination of the sum of T-2 and HT- 2 toxins in wheat [2]. The aim of present work was to evaluate the applicability of the above mentioned FP immunoassay to other unprocessed cereals, such as rye, and cereal-based products, such as oats crispbread, for the quantitative determination of the total content of T-2 and HT-2. The concept of determining the total content of T-2 and HT-2 in cereal samples for both official control purposes and risk assessment studies results in line with the EC Recommendation [3]. Ground samples (25 g) were weighed into a blender jar, added with 2.5 g of NaCl and extracted with 100 mL methanol/water 90:10 (v/v) by blending at high speed for 3 min. The extract was filtered through filter paper. No purification step of extracts was required, although in order to reduce the matrix effect a dilution step with NaCl solution (4 % for rye and 1% for oats crispbread), in a ratio 1:5 (v/v), was necessary to let precipitation of proteins and matrix interfering compounds. The diluted extract was then filtered through a glass microfibre filter and analysed by FP immunoassay. The evaluation of matrix effect in the FP measurements was performed, by comparing the regression line obtained with T-2/ HT-2 standard solutions in the range 0.5-7.5 ng/mL with the regression lines obtained by adding spiked diluted extracts of uncontaminated rye and oats crispbread at 5, 10 and 12 mg of analyzed matrix equivalent by means of parallelism and position statistical tests. No significant differences were observed between slopes (tcalc
Databáze: OpenAIRE
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