Drosophila S2 cells secrete wingless on exosome-like vesicles but the wingless gradient forms independently of exosomes

Autor: Beckett, Karen, Monier, Solange, Palmer, Lucy, Alexandre, Cyrille, Green, Hannah, Bonneil, Eric, Raposo, Graca, Thibault, Pierre, Le Borgne, Roland, Vincent, Jean-Paul
Přispěvatelé: Division of Developmental Biology, MRC National Institute for Medical Research, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Immunologie et en Cancérologie [UdeM-Montréal] (IRIC), Université de Montréal (UdeM), Compartimentation et dynamique cellulaires (CDC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Medical Research Council (MRC grant U117584268), Marie Curie International Re-integration Grant, Wellcome Project Grant, ATIP programme CNRS, Région Bretagne (Programme ACOMB 'Notasid' no 2168), INCA., Le Corre, Morgane, Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Traffic
Traffic, 2013, 14 (1), pp.82-96. ⟨10.1111/tra.12016⟩
Traffic, Wiley, 2013, 14 (1), pp.82-96. ⟨10.1111/tra.12016⟩
ISSN: 1398-9219
1600-0854
DOI: 10.1111/tra.12016⟩
Popis: International audience; Wingless acts as a morphogen in Drosophila wing discs, where it specifies cell fates and controls growth several cell diameters away from its site of expression. Thus, despite being acylated and membrane associated, Wingless spreads in the extracellular space. Recent studies have focussed on identifying the route that Wingless follows in the secretory pathway and determining how it is packaged for release. We have found that, in medium conditioned by Wingless-expressing Drosophila S2 cells, Wingless is present on exosome-like vesicles and that this fraction activates signal transduction. Proteomic analysis shows that Wingless-containing exosome-like structures contain many Drosophila proteins that are homologous to mammalian exosome proteins. In addition, Evi, a multipass transmembrane protein, is also present on exosome-like vesicles. Using these exosome markers and a cell-based RNAi assay, we found that the small GTPase Rab11 contributes significantly to exosome production. This finding allows us to conclude from in vivo Rab11 knockdown experiments, that exosomes are unlikely to contribute to Wingless secretion and gradient formation in wing discs. Consistent with this conclusion, extracellularly tagged Evi expressed from a Bacterial Artificial Chromosome is not released from imaginal disc Wingless-expressing cells.
Databáze: OpenAIRE
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