Effect of High-Fat Diet and its Reversal on the Thoracic Duct Lymph Composition in Pigs

Autor: Szuba, A., Gomulkiewicz, A., Janczak, D., Grzegorek, I., Jablonska, K., Drozdz, K., Chmielewska, M., Piotrowska, A., Paslawski, R., Paslawska, U., Janiszewski, A., Dziegiel, P., Gerard, Nicolas, Thien, Séverine, Verny, Marie-Anne, Mazur, André
Přispěvatelé: Regional Specialist Hospital, Partenaires INRAE, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Wrovasc-project, European Regional Development Fund, Centre de Recherche en Nutrition Humaine (CRNH). FRA.
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: 6ème Journée scientifique du CNRH Auvergne. 2013; 6. Journée scientifique du CNRH Auvergne, Clermont-Ferrand, FRA, 30-30
6. Journée scientifique du CNRH Auvergne
6. Journée scientifique du CNRH Auvergne, 2013, Clermont-Ferrand, France., 2013, 6ème Journée scientifique du CNRH Auvergne
6. Journée scientifique du CNRH Auvergne, 2013, Clermont-Ferrand, France. 2013, 6ème Journée scientifique du CNRH Auvergne
Popis: Présentation poster : Mazur A.; National audience; High-fat, carbohydrate and low fiber diet is linked to increased CV risk as demonstrated in both experimental animals and epidemiological studies. Reversal to the healthy diet is known to decrease CV risk. Lymph transports both lipids absorbed in intestine and cholesterol from tissues (reverse cholesterol transport). Lipid composition of both post-nodal and pre-nodal lymph is affected by several pathophysiological conditions. Thus, the aim of the presents study was to evaluate how long-term changes in dietary fat intake in pigs alter the lymph lipid and lipoproteins. MATERIALS AND METHODS: Thirty-two female pigs were divided into three experimental groups: Group 1 - control: regular diet (RD - low fat – 3%) for 12 months; Group 2 – increased fat diet ad libitum (HFD) for 12 months (first 6 months moderate-fat (7.5%) diet and following 6 months high-fat diet (16% fat); Group 3: Reversal diet (HRD): HFD for 9 months followed by RD for 3 months. Pigs were examined every 3 months for: body weight, blood pressure, lipidemia, arterial stiffness and elasticity, intima-media complex (IMC) measured by ultrasound. After 12 months on the respective diets, all animals were killed after 24 hours fasting and thoracic duct lymph was collected. Samples from eight animals from each group were used for lymph lipid and lipoprotein distribution analysis by density gradient ultracentrifugation. RESULTS: Lipid lymph analysis revealed higher total cholesterol concentration in HFD fed animals than in these on the control diet. Lymph lipoprotein distribution showed that HFD caused an increase in chylomicron and HDL cholesterol levels, but did not affected VLDL and LDL cholesterol. The return from the HFD to the RD partly restored lymph cholesterol levels to values found in the control (RD) group. In conclusion, our findings support that the level of dietary fat affects lymphatic reverse cholesterol transport.
Databáze: OpenAIRE
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