Surface-modified functionalized polycaprolactone scaffolds for bone repair: In vitro and in vivo experiments

Autor: Jensen, Jonas, Rölfing, Jan Hendrik Duedal, Svend Le, Dang Quang, Kristiansen, Asger Albaek, Nygaard, Jens Vinge, Hokland, Lea Bjerre, Bendtsen, Michael, Kassem, Moustapha, Lysdahl, Helle, Bünger, Cody
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Jensen, J, Rolfing, J H D, Svend Le, D Q, Kristiansen, A A, Nygaard, J V, Bjerre Hokland, L, Bendtsen, M, Kassem, M, Lysdahl, H & Bunger, C E 2014, ' Surface-modified functionalized polycaprolactone scaffolds for bone repair: In vitro and in vivo experiments ', Journal of Biomedical Materials Research. Part A, vol. 102, no. 9, 24123983, pp. 2993-3003 . https://doi.org/10.1002/jbm.a.34970
Jensen, J, Rölfing, J H D, Svend Le, D Q, Kristiansen, A A, Nygaard, J V, Hokland, L B, Bendtsen, M, Kassem, M, Lysdahl, H & Bünger, C 2014, ' Surface-modified functionalized polycaprolactone scaffolds for bone repair : In vitro and in vivo experiments ', Journal of Biomedical Materials Research. Part A, vol. 102, no. 9, pp. 2993-3003 . https://doi.org/10.1002/jbm.a.34970
DOI: 10.1002/jbm.a.34970
Popis: A porcine calvaria defect study was carried out to investigate the bone repair potential of three-dimensional (3D)-printed poly-ε-caprolactone (PCL) scaffolds embedded with nanoporous PCL. A microscopic grid network was created by rapid prototyping making a 3D-fused deposition model (FDM-PCL). Afterward, the FDM-PCL scaffolds were infused with a mixture of PCL, water, and 1,4-dioxane and underwent a thermal-induced phase separation (TIPS) followed by lyophilization. The TIPS process lead to a nanoporous structure shielded by the printed microstructure (NSP-PCL). Sixteen Landrace pigs were divided into two groups with 8 and 12 weeks follow-up, respectively. A total of six nonpenetrating holes were drilled in the calvaria of each animal. The size of the cylindrical defects was h 10 mm and Ø 10 mm. The defects were distributed randomly using following groups: (a) NSP-PCL scaffold, (b) FDM-PCL scaffold, (c) autograft, (d) empty defect, (a1) NSP-PCL scaffold + autologous mononuclear cells, and (a2) NSP-PCL scaffold + bone morphogenetic protein 2. Bone volume to total volume was analyzed using microcomputed tomography (µCT) and histomorphometry. The µCT and histological data showed significantly less bone formation in the NSP-PCL scaffolds in all three variations after both 8 and 12 weeks compared to all other groups. The positive autograft control had significantly higher new bone formation compared to all other groups except the FDM-PCL when analyzed using histomorphometry. The NSP-PCL scaffolds were heavily infiltrated with foreign body giant cells suggesting an inflammatory response and perhaps active resorption of the scaffold material. The unmodified FDM-PCL scaffold showed good osteoconductivity and osseointegration after both 8 and 12 weeks.
Databáze: OpenAIRE
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