In situ application of non-thermal plasma: preliminary investigations for colorectal and lung tolerance
| Autor: | Vandamme, Marc, Robert, Eric, Sobilo, Julien, Sarron, Vanessa, Ries, Delphine, Dozias, Sébastien, Lerondel, Stéphanie, Legrain, Brigitte, Pape, Alain Le, Pouvesle, Jean-Michel |
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| Přispěvatelé: | POUVESLE, Jean-Michel, Groupe de recherches sur l'énergétique des milieux ionisés (GREMI), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Transgénèse et Archivage d'Animaux Modèles - Centre d'Imagerie du Petit Animal (TAAM-CIPA), Centre National de la Recherche Scientifique (CNRS), IPCS |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
[SDV] Life Sciences [q-bio]
[SPI]Engineering Sciences [physics] Atmospheric Plasma Effects [SPI] Engineering Sciences [physics] [SDV]Life Sciences [q-bio] [SPI.PLASMA]Engineering Sciences [physics]/Plasmas Plasma Jet Plasma Gun [SPI.PLASMA] Engineering Sciences [physics]/Plasmas Biomedical applications of plasma |
| Zdroj: | ISPC 20 ISPC 20, IPCS, Jul 2011, Philadelphie, United States |
| Popis: | International audience; Non-thermal plasma (NTP) has a significant antitumor activity in vitroon different cell lines including brain, colorectal and lung tumor. The necessarydose to kill 50% of cells was ~9J/cm² for these different cell lines. ReactiveOxygen Species (ROS) generated during treatment appears to be responsible ofthis effect and induce DNA damages together with a cell cycle arrest leading toan apoptosis induction. These promising results in vitro lead us to evaluate NTPin vivo. The tolerance study was conducted with NF-kB-luc reported mice. NFkBis a transcription factor implied in inflammatory process and bioluminescenceimaging of this strain of mice allows monitoring of inflammation intensity andtime course. Tracheal intubation with Plasma Gun using a small catheter and lowgas flow was successfully achieved. As a preliminary result, an induction of theinflammatory process was observed in the treated lung after 6 and 12 min oftreatment. Concerning colorectal inflammation following FE-DBD treatment,NF-kB mice failed to reveal any inflammation in preliminary experiments.Further investigations are needed to elaborate a treatment protocol for lung andcolorectal tumor treatment.Keywords: Non-Thermal Plasma, Antitumor activity, Tolerance study |
| Databáze: | OpenAIRE |
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