Hydrostatic pressure effects membrane and storage lipid compositions of the piezotolerant hydrocarbon-degrading Marinobacter hydrocarbonoclasticus strain #5

Autor: Grossi, Vincent, Yakimov, Michail M., Al Ali, Badr, Tapilatu, Yosmina, CUNY, Philippe, Goutx, Madeleine, La Cono, Violetta, Giuliano, Laura, TAMBURINI, Christian
Přispěvatelé: PaleoEnvironnements et PaleobioSphere (PEPS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Insitute for Coastal Marine Environment, Consiglio Nazionale delle Ricerche (CNR), Laboratoire de MicrobiologiE de Géochimie et d'Ecologie Marines (LMGEM), Centre National de la Recherche Scientifique (CNRS)-Université de la Méditerranée - Aix-Marseille 2, Mediterranean Science Commission, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), European Science Foundation : MIDDLE 06-EuroDEEP-FP-004, CNRS-INSU : BIOHYDEX EC2CO, National Council of Research (CNR)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Environmental Microbiology
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2010, 12(7), pp.2020-2033. ⟨10.1111/j.1462-2920.2010.02213.x⟩
Environmental Microbiology, 2010, 12(7), pp.2020-2033. ⟨10.1111/j.1462-2920.2010.02213.x⟩
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2010, 12 (7), pp.2020-2033. ⟨10.1111/j.1462-2920.2010.02213.x⟩
Environmental Microbiology, 2010, 12 (7), pp.2020-2033. ⟨10.1111/j.1462-2920.2010.02213.x⟩
ISSN: 1462-2912
1462-2920
Popis: International audience; A new piezotolerant alkane-degrading bacterium (Marinobacter hydrocarbonoclasticus strain #5) was isolated from deep (3475 m) Mediterranean seawater and grown at atmospheric pressure (0.1 MPa) and at 35 MPa with hexadecane as sole source of carbon and energy. Modification of the hydrostatic pressure influenced neither the growth rate nor the amount of degraded hexadecane 90%) during 13 days of incubation. However, the lipid composition of the cells sharply differed under both pressure conditions. At 0.1 MPa, M. hydrocarbonoclasticus #5 biosynthesized large amounts 62% of the total cellular lipids) of hexadecane-derived wax esters (WEs), which accumulated in the cells under the form of individual lipid bodies. Intracellular WEs were also synthesized at 35 MPa, but their proportion was half that at 0.1 MPa. This lower WE content at high pressure was balanced by an increase in the total cellular phospholipid content. The chemical composition of WEs formed under both pressure conditions also strongly differed. Saturated WEs were preferentially formed at 0.1 MPa whereas diunsaturated WEs dominated at 35 MPa. This increase of the unsaturation ratio of WEs resembled the one classically observed for bacterial membrane lipid homeostasis. Remarkably, the unsaturation ratio of membrane fatty acids of M. hydrocarbonoclasticus grown at 35 MPa was only slightly higher than at 0.1 MPa. Overall, the results suggest that intracellular WEs and phospholipids play complementary roles in the physiological adaptation of strain #5 to different hydrostatic pressures.
Databáze: OpenAIRE
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