The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

Autor:	Mir Morro, Bartomeu, Solés, X., González, Carlos, Escaja Sánchez, Nuria
Přispěvatelé:	Ministerio de Economía y Competitividad (España), Universitat de Barcelona
Rok vydání:	2017
Předmět:	endocrine system diseases Science Àcids nucleics digestive oral and skin physiology Oligonucleotides Oligonucleòtids digestive system digestive system diseases Nucleic acids Medicine Citidina difosfat de colina Cytidine diphosphate choline
Zdroj:	Digital.CSIC. Repositorio Institucional del CSIC instname Dipòsit Digital de la UB Universidad de Barcelona Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) Recercat. Dipósit de la Recerca de Catalunya
Popis:	Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C or psC:psC hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::80e58cb76d53c62692c49a022f5752f8 http://hdl.handle.net/10261/165896 Zobrazit plný text záznamu