Quantification of GABA, Glutamate and Glutamine in a single Measurement at 3T using GABA-edited MEGA-PRESS

Autor: Nezhad, Faezeh Sanaei, Anton, Adriana, Michou, Emilia, Jung, JeYoung, Parkes, Laura M., Williams, Stephen R.
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Nezhad, F S, Anton, A, Michou, E, Jung, J, Parkes, L M & Williams, S R 2018, ' Quantification of GABA, Glutamate and Glutamine in a single Measurement at 3T using GABA-edited MEGA-PRESS ', NMR in Biomedicine, vol. 31, no. 1, e3847 . https://doi.org/10.1002/nbm.3847
Sanaei Nezhad, F, Anton, A, Michou, E, Jung, J, Parkes, L M & Williams, S R 2018, ' Quantification of GABA, glutamate and glutamine in a single measurement at 3 T using GABA-edited MEGA-PRESS ' NMR in Biomedicine, vol 31, no. 1 . DOI: 10.1002/nbm.3847
ISSN: 0952-3480
1099-1492
DOI: 10.1002/nbm.3847
Popis: γ-Aminobutyric acid (GABA) and glutamate (Glu), major neurotransmitters in the brain, are recycled through glutamine (Gln). All three metabolites can be measured by magnetic resonance spectroscopy in vivo, although GABA measurement at 3 T requires an extra editing acquisition, such as Mescher-Garwood point-resolved spectroscopy (MEGA-PRESS). In a GABA-edited MEGA-PRESS spectrum, Glu and Gln co-edit with GABA, providing the possibility to measure all three in one acquisition. In this study, we investigated the reliability of the composite Glu + Gln (Glx) peak estimation and the possibility of Glu and Gln separation in GABA-edited MEGA-PRESS spectra. The data acquired in vivo were used to develop a quality assessment framework which identified MEGA-PRESS spectra in which Glu and Gln could be estimated reliably. Phantoms containing Glu, Gln, GABA and N-acetylaspartate (NAA) at different concentrations were scanned using GABA-edited MEGA-PRESS at 3 T. Fifty-six sets of spectra in five brain regions were acquired from 36 healthy volunteers. Based on the Glu/Gln ratio, data were classified as either within or outside the physiological range. A peak-by-peak quality assessment was performed on all data to investigate whether quality metrics can discriminate between these two classes of spectra. The quality metrics were as follows: the GABA signal-to-noise ratio, the NAA linewidth and the Glx Cramer-Rao lower bound (CRLB). The Glu and Gln concentrations were estimated with precision across all phantoms with a linear relationship between the measured and true concentrations: R1 = 0.95 for Glu and R1 = 0.91 for Gln. A quality assessment framework was set based on the criteria necessary for a good GABA-edited MEGA-PRESS spectrum. Simultaneous criteria of NAA linewidth
Databáze: OpenAIRE
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