Cell-free synthetic biology for in vitro prototype engineering
| Autor: | Moore, Simon J., MacDonald, James T., Freemont, Paul S. |
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| Přispěvatelé: | Engineering & Physical Science Research Council (E |
| Rok vydání: | 2017 |
| Předmět: |
EXPRESSION
Biochemistry & Molecular Biology cell free Science & Technology Cell-Free System REGULATORY ELEMENTS MEMBRANE-PROTEINS COMPONENTS prototyping 0601 Biochemistry And Cell Biology TRANSCRIPTION-TRANSLATION SYSTEM GENE NETWORKS FREE PROTEIN-SYNTHESIS Biosynthetic Pathways modelling RNA-POLYMERASE MINIMAL CELL E-COLI gene expression 1101 Medical Biochemistry And Metabolomics Gene Regulatory Networks Synthetic Biology Life Sciences & Biomedicine |
| ISSN: | 0300-5127 |
| Popis: | Cell-free transcription – translation is an expanding fi eld in synthetic biology as a rapid prototyping platform for blueprinting the design of synthetic biological devices. Exemplar efforts include translation of prototype designs into medical test kits for on-site identi fi ca- tion of viruses (Zika and Ebola), while gene circuit cascades can be tested, debugged and re-designed within rapid turnover times. Coupled with mathematical modelling, this discipline lends itself towards the precision engineering of new synthetic life. The next stages of cell-free look set to unlock new microbial hosts that remain slow to engineer and unsuited to rapid iterative design cycles. It is hoped that the development of such systems will provide new tools to aid the transition from cell-free prototype designs to functioning synthetic genetic circuits and engineered natural product pathways in living cells. |
| Databáze: | OpenAIRE |
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