Identifying genetic networks underlying myometrial transition to labor
| Autor: | Salomonis, N., Cotte, N., Zambon, A. C., Pollard, K. S., Vranizan, K., Doniger, S. W., Dolganov, G., Bruce Conklin |
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| Rok vydání: | 2005 |
| Předmět: |
RNA Processing
Bioinformatics 1.1 Normal biological development and functioning Messenger Post-Transcriptional Gestational Age Mice Uterine Contraction Genetic Underpinning research Pregnancy Information and Computing Sciences Genetics 2.1 Biological and endogenous factors Animals Cluster Analysis Aetiology Gene Expression Profiling Postpartum Period Parturition Biological Sciences Heterotrimeric GTP-Binding Proteins Kinetics Good Health and Well Being Gene Expression Regulation Myometrium RNA Female Transcription Environmental Sciences Signal Transduction |
| Zdroj: | Genome biology, vol 6, iss 2 Scopus-Elsevier Salomonis, N; Cotte, N; Zambon, AC; Pollard, KS; Vranizan, K; Doniger, SW; et al.(2005). Identifying genetic networks underlying myometrial transition to labor. GENOME BIOLOGY, 6(2). doi: 10.1186/gb-2005-6-2-r12. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/66r471mg |
| DOI: | 10.1186/gb-2005-6-2-r12. |
| Popis: | BackgroundEarly transition to labor remains a major cause of infant mortality, yet the causes are largely unknown. Although several marker genes have been identified, little is known about the underlying global gene expression patterns and pathways that orchestrate these striking changes.ResultsWe performed a detailed time-course study of over 9,000 genes in mouse myometrium at defined physiological states: non-pregnant, mid-gestation, late gestation, and postpartum. This dataset allowed us to identify distinct patterns of gene expression that correspond to phases of myometrial 'quiescence', 'term activation', and 'postpartum involution'. Using recently developed functional mapping tools (HOPACH (hierarchical ordered partitioning and collapsing hybrid) and GenMAPP 2.0), we have identified new potential transcriptional regulatory gene networks mediating the transition from quiescence to term activation.ConclusionsThese results implicate the myometrium as an essential regulator of endocrine hormone (cortisol and progesterone synthesis) and signaling pathways (cyclic AMP and cyclic GMP stimulation) that direct quiescence via the transcriptional upregulation of both novel and previously associated regulators. With term activation, we observe the upregulation of cytoskeletal remodeling mediators (intermediate filaments), cell junctions, transcriptional regulators, and the coordinate downregulation of negative control checkpoints of smooth muscle contractile signaling. This analysis provides new evidence of multiple parallel mechanisms of uterine contractile regulation and presents new putative targets for regulating myometrial transformation and contraction. |
| Databáze: | OpenAIRE |
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