Gonadal regulation of European sea bass defensin beta genes expression upon NNV infection and vaccination
| Autor: | Cervera, L. (Laura), Arizcun-Arizcun, M. (Marta), Cuesta, A. (Alberto), Chaves-Pozo, E. (Elena) |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Popis: | Aquaculture is one of the most prosperous economic sectors. Nevertheless, the natural outbreaks of several infectious diseases make the sector to deal with important economic losses. One of the most important pathogens in the Mediterranean Sea is nodavirus (NNV). NNV is the agent causing viral encephalopathy and retinopathy in more than 170 fish species including some of the most impact in Spanish hatcheries as European sea bass (Dicentrarchus labrax). Antimicrobial peptides (AMPs) are short aminoacidic sequences which constitute important mediators of the innate immune response in teleost fish. AMPs can kill directly a broad range of pathogens such as bacteria or viruses and modulate the host immune response leading to a more effective clearance of pathogens. These properties along with the world emergency in antimicrobial resistance (AMR) make AMPs good candidates to replace traditional antimicrobials. Therefore, we aimed to analyze the molecular regulation of European sea bass defensin beta genes upon NNV infection and after NNV vaccination. To achieve our objective, European sea bass were infected with NNV and samples of head-kidney (HK), brain and gonad were taken. In addition, gonadal cells from healthy males were in vitro stimulated with NNV. Then, ovary samples of control females or vaccinated against NNV (pBAD vaccine) were taken, as well as fertilized eggs and larvae from the same groups. To support our data, we also analyzed in silico the potential antiviral activity of the protein encoded by the studied genes. Our results show that defensin beta 1 gene is up-regulated upon in vivo NNV infection even if in the in silico study showed the lesser predicted activity. Interestingly, the in vitro NNV challenge resulted in no variation of the defensin beta 1 gene, while defensin beta 2.1 and 2.2 genes were blocked upon this stimulus. Strikingly, females vaccinated with pBAD greatly down-regulate defensin beta 2.2 expression. Moreover, larvae from vaccinated mother up-regulate all defensin beta genes. In conclusion, defensin beta 1 gene seems to be involved in the defense against viruses while defensin beta 2 genes appear to possess a more specific function in gonad. Evaluación de los efectos de las microalgas, incluidas en dietas de dorada, sobre el crecimiento, supervivencia y resistencia a infecciones. ALGAFISH |
| Databáze: | OpenAIRE |
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