Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana

Autor: Rasul , Sumaira, Dubreuil , Carole, Lamotte , Olivier, Koen , Emmanuel, Poinssot , Benoît, Alcaraz , Gérard, Wendehenne , David, Jeandroz , Sylvain
Přispěvatelé: Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Plant, Cell and Environment
Plant, Cell and Environment, Wiley, 2012, 35 (8), pp.1483-1499. ⟨10.1111/j.1365-3040.2012.02505.x⟩
Plant, Cell and Environment 8 (35), 1483-1499. (2012)
Plant, Cell and Environment, Wiley, 2012, 35 (8), pp.1483-1499. 〈10.1111/j.1365-3040.2012.02505.x〉
ISSN: 0140-7791
1365-3040
DOI: 10.1111/j.1365-3040.2012.02505.x⟩
Popis: Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in [i]Arabidopsis thaliana[/i] leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with [i]Botrytis cinerea[/i]. We provided evidence that OGs triggered a fast and long lasting NO production which was Ca2+ dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L-NAME. Intriguingly, we showed that L-NAME affected NO production by interfering with NR activity, thus questioning the mechanisms of how this compound impairs NO synthesis in plants. We further demonstrated that NO modulates RBOHD-mediated reactive oxygen species (ROS) production and participates in the regulation of OG-responsive genes such as anionic peroxidase (PER4) and a beta-1,3-glucanase. Mutant plants impaired in PER4 and beta-1,3-glucanase, as well as Col-0 plants treated with the NO scavenger cPTIO, were more susceptible to [i]B. cinerea.[/i] Taken together, our investigation deciphers part of the mechanisms linking NO production, NO-induced effects and basal resistance to[i] B. cinerea[/i]
Databáze: OpenAIRE
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