The effects of Panax Ginseng root extract on carbohydrate and lipid disturbances associated with alloxan-induced diabetic rats

Autor: Naseem, M., Nazih, El-Hassane, Ouguerram, Khadija, Rabbani, Imtiaz, Zaneb, Hafsa, Rehman, H. U., Masood, I., Huvelin, J. M., Yousaf, M. S., Tahir, S. K.
Přispěvatelé: Department of Zoology, University of Balochistan, Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11), Physiologie des Adaptations Nutritionnelles (PhAN), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Department of Physiology, University of São Paulo (USP), Department of Anatomy and Histology, The University of Sydney, Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Higher Education Commission (HEC), Pakistan, Laboratoire de Biochimie, MMS, Nantes, France, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Saclay, Physiologie des Adaptations Nutritionnelles [UMR_A1280] (PhAN)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of Animal and Plant Sciences
Journal of Animal and Plant Sciences, Elewa BioSciences, 2016, 26 (5), pp.1218-1225
Journal of Animal and Plant Sciences, 2016, 26 (5), pp.1218-1225
ISSN: 2071-7024
1018-7081
Popis: Diabetes is a major public health issue. Panax ginseng is an anciently used herbal drug for diabetic treatment. Antidiabetic effects of P. ginseng are attributed to ginsenosides. The objective of current study is to determine the antidiabetic effects of Panax ginseng root extract at gene level. Rats were fed on high-fatty diet for two weeks and divided into three groups (8rats/group): Non-diabetic control group (NDG), Diabetic-group (DG), Diabetic+300mg/kg Panax ginseng root extract group (DM+PGE). On 14th day, the rats were kept in overnight-fasting and administered single intraperitoneal injection of alloxan-monohydrate dissolved in 0.5ml of saline solution at the dose of 120-130mg/Kg body weight (BW). BW and blood glucose were measured on 1st and 14th week. After 14 weeks, fasting/basal blood samples were collected for the biochemical analysis. Liver, skeletal muscle and adipose tissue were also collected for mRNA genes expression of Glucose transporter-4 (GLUT-4), Insulin receptor substrate-1(IRS-1), Insulin receptor (IR), Sterol regulatory element binding protein-1c (SREBP-1c), Fatty acid sythase (FAS), Peroxisome proliferator-activated receptor-alpha (PPAR-alpha), Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and tumor necrosis factor-alpha (TNF-alpha). We found a significant reduction in the BW of DM+PGE group at 14th week. Glycemia was significantly higher in DG and significant reduction was recorded in DM+PGE group. While serum glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatinine levels decreased significantly in treated groups. We observed significant increase for catalase (CAT) and decrease for malondialdehyde (MDA) in DM+PGE group in comparison to DG. PGE showed significant increase for HDL-C and decrease for TG. PGE significantly down-regulate hepatic TNF-alpha. For skeletal muscle, we recorded significant up-regulation for GLUT-4 and PPAR-alpha while for adipose tissue, we measured upregulation for IRS-1 and PPAR-gamma and significant down-regulation was recorded for TNF-alpha. In conclusion, PGE has strong anti-diabetic effects mediated by a modulating effect on involved key genes.
Databáze: OpenAIRE
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