Cost-efficient sugar-based cellulase production
| Autor: | Simo Ellilä, Lauri Kujanpää, Kaisa Marjamaa, Toni Paasikallio, Markku Saloheimo, Nina Aro |
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| Přispěvatelé: | Hytonen, Eemeli, Vepsalainen, Jessica |
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| Zdroj: | Scopus-Elsevier VTT Technical Research Centre of Finland-PURE Ellilä, S, Kujanpää, L, Marjamaa, K, Paasikallio, T, Saloheimo, M & Aro, N 2018, Cost-efficient sugar-based cellulase production . in E Hytonen & J Vepsalainen (eds), NWBC 2018 : Proceedings of the 8th Nordic Wood Biorefinery Conference . VTT Technical Research Centre of Finland, VTT Technology, no. 340, pp. 185-192, 8th Nordic Wood Biorefinery Conference, NWBC 2018, Helsinki, Finland, 23/10/18 . |
| Popis: | Enzymes remain a significant operational cost in biochemical biorefining processes. Inexpensive streams of sugar are available at many potential biorefining sites, and these could be an optimal raw material for the on-site production of cellulase enzymes. However, simple sugars do not support cellulase production using conventional cellulase-producing strains, necessitating either process or strain improvements. Here we describe improved glucose-based enzyme production processes enabling industrially relevant cellulase production kinetics. Extracellular protein titers of up to 53 g/L could be reached in under 93 hours, representing an up to 260% improvement in productivity over previously reported results. Glucose-produced enzyme is also shown to perform well on pre-treated corn stover. Using the NREL biorefinery model, enzyme production costs are estimated to be $2,8/kg protein or 6/L produced ethanol. Additionally, we briefly discuss how organic nitrogen could be cycled in enzyme production, and how the described processes could fit into existing and emerging biorefining operations. |
| Databáze: | OpenAIRE |
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