Next generation Potentiostat-on-a-Disc (PoD) for electrochemical sensing on disc
| Autor: | Sriram Thoppe Rajendran, Hamed Shamkhalichenar, Roman Slipets, Jin-Woo Choi, Kinga Zór, Anja Boisen |
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| Zdroj: | Technical University of Denmark Orbit Thoppe Rajendran, S, Shamkhalichenar, H, Slipets, R, Choi, J-W, Zór, K & Boisen, A 2020, ' Next generation Potentiostat-on-a-Disc (PoD) for electrochemical sensing on disc ', 30th Anniversary World Congress on Biosensors, Busan, Korea, Republic of, 26/05/2020-29/05/2020 . |
| Popis: | Reliable, cost-effective point-of-need sensing tools are required for biomedical diagnostics, food safety and environmental monitoring. The compactness of Lab-on-a-Disc (LoD) platforms facilitates the development of portable, sample-to-answer detection units1 and the combination of electrochemical sensing with microfluidics is advantageous (e.g. miniaturization and ease of integration of sensor and detection unit)2. However, electrochemical detection in LoD is challenging due to the need for robust interfacing between electrodes and potentiostat for real-time measurement during spin3. This can be partially overcome with sliprings, but the inherent noise due to movement of contact points during rotation is inevitable4. The wirelessly powered potentiostat-on-a-disc (PoD)5 combined with LoD solved the above mentioned noise issues, however there is still a necessity for further simplification of the PoD for increasing robustness and ease-of-use.The next generation PoD (v2.0) was significantly simplified by using off-the-shelf components (Fig. 1)and consists of a core circuit (Fig. 1a) and a shield that connects the electrodes to the PoD (Fig. 2a). The data recorded with a custom-made interface was transferred via Bluetooth. A digital gain selector and Li-ion battery was also implemented to increase the compactness, portability and operational simplicity. As a case study the PoD v2.0 was used for the detection of ascorbic acid, an antioxidant found in various foodproducts and often measured from blood6. The potentiostat was connected to electrodes on disc (Fig. 2b) and the amperometric response for successive addition of ascorbic acid was recorded (Fig. 2c). The obtained calibration curve is shown in Fig. 1d, with detection limit of 32 µM.Considering the user-friendliness and versatility of the PoD v2.0, as a next step, we aim for full integration of blood processing on the LoD device for sample-to-answer analysis of e.g. methotrexate and irinotecan for therapeutic drug monitoring. |
| Databáze: | OpenAIRE |
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