Differential effects of 17 alpha-ethinylestradiol on the neutral and acidic pathways of bile salt synthesis in the rat

Autor:	Koopen, Nr, Post, Sm, Wolters, H., Havinga, R., Stellaard, F., Boverhof, R., Kuipers, F., Hans Princen
Přispěvatelé:	Groningen University Institute for Drug Exploration (GUIDE), Center for Liver, Digestive and Metabolic Diseases (CLDM)
Předmět:	BILIARY DRAINAGE CHOLIC-ACID FOOD-INTAKE LIVER sterol-27-hydroxylase bile METABOLISM CHOLESTEROL 7-ALPHA-HYDROXYLASE ETHINYL ESTRADIOL enterohepatic circulation LIPOPROTEIN RECEPTOR Delta(22)-beta-muri-cholate cholesterol-7 alpha-hydroxylase cholestasis STEROL 27-HYDROXYLASE MESSENGER-RNA estrogens
Zdroj:	University of Groningen Web of Science Journal of Lipid Research, 40(1), 100-108. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
ISSN:	0022-2275
Popis:	Effects of 17 alpha-ethinylestradiol (EE) on the neutral and acidic biosynthetic pathways of bile salt (BS) synthesis were evaluated in rats with an intact enterohepatic circulation and in rats with long-term bile diversion to induce BS synthesis, For this purpose, bile salt pool composition, synthesis of individual BS in vivo, hepatic activities, and expression levels of cholesterol 7 alpha-hydroxylase (CYP7A), and sterol 27-hydroxylase (CYP27), as well as of other enzymes involved in BS synthesis, were analyzed in rats treated with EE (5 mg/kg, 3 days) or its vehicle, BS pool size was decreased by 27% but total BS synthesis was not affected by EE in intact rats. Synthesis of cholate was reduced by 68% in EE-treated rats, while that of chenodeoxycholate was increased by 60%, The recently identified Delta(22)-isomer of beta-muricholate contributed for 5.4% and 18.3% (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::7424d90cee34807f78120de63a89b5f1 https://research.rug.nl/en/publications/dc9d2df4-1ba5-4dd4-975c-f77c2cbd5128 Zobrazit plný text záznamu