Harmonization of the quantitative determination of volatile fatty acids profile in aqueous matrix samples by direct injection using GC and HPLC techniques: Multi-laboratory validation study

Autor: Raposo, Francisco, Borja, Rafael, Cacho, Jesús A., Mumme, Jan, Battimelli, Audrey, Bolzonella, David, Schuit, Anthony D., Noguerol-Arias, Joan, Frigon, Jean-Claude, Peñuela, Gustavo A., Muehlenberg, Jana, Sambusiti, Cécilia
Přispěvatelé: Instituto de la Grasa, Veolia Environnement, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Department of Biotechnology, University of Verona (UNIVR), Delft University of Technology (TU Delft), Institute of Agrifood Research and Technology (IRTA), National Research Council of Canada (NRC), Universidad de Antioquia = University of Antioquia [Medellín, Colombia], Deutsches Biomasseforschungszentrum (DBFZ), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Journal of Chromatography A
Journal of Chromatography A, Elsevier, 2015, 1413, pp.94-106. ⟨10.1016/j.chroma.2015.08.008⟩
ISSN: 0021-9673
1873-3778
DOI: 10.1016/j.chroma.2015.08.008⟩
Popis: International audience; The performance parameters of volatile fatty acids (VFAs) measurements were assessed for the first time by a multi-laboratory validation study among 13 laboratories. Two chromatographic techniques (GC and HPLC) and two quantification methods such as external and internal standard (ESTD/ISTD) were combined in three different methodologies GC/ESTD, HPLC/ESTD and GC/ISTD. Linearity evaluation of the calibration functions in a wide concentration range (10–1000 mg/L) was carried out using different statistical parameters for the goodness of fit. Both chromatographic techniques were considered similarly accurate. The use of GC/ISTD, despite showing similar analytical performance to the other methodologies, can be considered useful for the harmonization of VFAs analytical methodology taking into account the normalization of slope values used for the calculation of VFAs concentrations. Acceptance criteria for VFAs performance parameters of the multi-laboratory validation study should be established as follows: (1) instrument precision (RSDINST ≤ 1.5%); (2) linearity (R2 ≥ 0.998; RSDSENSITIVITY ≤ 4%; REMAX ≤ 8%; REAVER ≤ 3%); (3) precision (RSD ≤ 1.5%); (4) trueness (recovery of 97–103%); (5) LOD (≤3 mg/L); and (6) LOQ (10 mg/L).
Databáze: OpenAIRE
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