Visible light crosslinkable human hair keratin hydrogels
| Autor: | Yue, K, Liu, Y, Byambaa, B, Singh, V, Liu, W, Li, X, Sun, Y, Zhang, YS, Tamayol, A, Zhang, P, Ng, KW, Annabi, N, Khademhosseini, A |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Bioengineering & translational medicine, vol 3, iss 1 Yue, K; Liu, Y; Byambaa, B; Singh, V; Liu, W; Li, X; et al.(2018). Visible light crosslinkable human hair keratin hydrogels.. Bioengineering & Translational Medicine, 3(1), 37-48. doi: 10.1002/btm2.10077. UCLA: Retrieved from: http://www.escholarship.org/uc/item/1z64f7xq |
| DOI: | 10.1002/btm2.10077. |
| Popis: | Keratins extracted from human hair have emerged as a promising biomaterial for various biomedical applications, partly due to their wide availability, low cost, minimal immune response, and the potential to engineer autologous tissue constructs. However, the fabrication of keratin-based scaffolds typically relies on limited crosslinking mechanisms, such as via physical interactions or disulfide bond formation, which are time-consuming and result in relatively poor mechanical strength and stability. Here, we report the preparation of photocrosslinkable keratin-polyethylene glycol (PEG) hydrogels via the thiol-norbornene "click" reaction, which can be formed within one minute upon irradiation of visible light. The resulting keratin-PEG hydrogels showed highly tunable mechanical properties of up to 45 kPa in compressive modulus, and long-term stability in buffer solutions and cell culture media. These keratin-based hydrogels were tested as cell culture substrates in both two-dimensional surface seeding and three-dimensional cell encapsulation, demonstrating excellent cytocompatibility to support the attachment, spreading, and proliferation of fibroblast cells. Moreover, the photocrosslinking mechanism makes keratin-based hydrogel suitable for various microfabrication techniques, such as micropatterning and wet spinning, to fabricate cell-laden tissue constructs with different architectures. We believe that the unique features of this photocrosslinkable human hair keratin hydrogel promise new opportunities for their future biomedical applications. |
| Databáze: | OpenAIRE |
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