Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
| Autor: | Pishbin, Fatemehsadat, Mouriño, Viviana Silvia Lourdes, Flor, Sabrina Andrea, Kreppel, Stefan, Salih, Vehid, Ryan, Mary P., Boccaccini, Aldo R. |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| DOI: | 10.1021/am5014166 |
| Popis: | Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings. Fil: Pishbin, Fatemehsadat. Imperial College London; Reino Unido Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kreppel, Stefan. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania Fil: Salih, Vehid. University College London; Estados Unidos Fil: Ryan, Mary P.. Imperial College London; Reino Unido Fil: Boccaccini, Aldo R.. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania |
| Databáze: | OpenAIRE |
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