Impact of polyphenols on the microbiota-gut-brain axis -Study of 5-O-caffeoylquinic acid and its gut bacterial-derived metabolites
| Autor: | Balaj, Gentiana, Tamanai-Shacoori, Zohreh, Bousarghin, Latifa, David-Le Gall, Sandrine, Guyot, Sylvain, Tomasi, Sophie, Nebija, Dashnor, Abasq, Marie-Laurence |
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| Přispěvatelé: | Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Faculty of Medicine, Department of Pharmacy, University of Prishtina, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Guyot, Sylvain |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | EDUC (European Digital UniverCity)-Lifelong Health and Wellbeing scientific seminar EDUC (European Digital UniverCity)-Lifelong Health and Wellbeing scientific seminar, Mar 2021, Pécs, Hungary |
| Popis: | International audience; Aim: Polyphenols, dietary compounds, have drawn increasing attention due to their ability to lower oxidative stress involved in many pathologies and due to their positive effects in modulation of redox cellular signalling pathways [1]. The objectives of our studies are to identify the compounds derived from polyphenols after cellular metabolization (neuronal cells and gut bacteria) and to highlight how these molecules can act beneficially on microbiota and prevent neurodegenerescence diseases [2]. A particular attention is paid on oxidized phenolic metabolites and their biological effects. Methods: Currently, we focus our work in the impact of 5-O-caffeoylquinic acid (5-CQA), a dietary phenolic compound with neuroprotective properties [3], on the growth and the metabolism of three intestinal bacterial species (Lactobacillus reuteri, Bacteroides fragilis and Bifidobacterium longum). In parallel, we determine the bioconversion of 5-CQA by these three bacteria in mono and co-cultures. We particularly use LC-ESI-MS 2 and a molecular networking approach (GNPS platform) [4] to identify the biotransformed metabolites (e.g. oxidized derivatives). In addition, electrochemical technics are helpful to study the redox mechanism of 5-CQA and to generate oxidized samples. Results: L. reuteri has shown capacity to biotransform 5-CQA notably into caffeic acid and its oxidized derivative quinone, and into 3-hydroxybenzoic acid, contrary to B. fragilis and B. longum which didn't show any ability. First experiments in co-cultures were also carried out. Conclusion and perspectives: We have demonstrated an oxidative pathway in the bioconversion of 5-CQA by L. reuteri. Further experiments will be carried out to show the role of native, oxidized and biotransformed dietary polyphenols in the gut-brain axis. |
| Databáze: | OpenAIRE |
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