Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
| Autor: | Zhang, Hongtao, Palma, Angelina S., Zhang, Yibing, Childs, Robert A., Liu, Yan, Mitchell, Daniel A., Guidolin, Leticia Soledad, Weigel, Wilfried, Mulloy, Barbara, Ciocchini, Andres Eduardo, Feizi, Ten, Chai, Wengang |
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| Přispěvatelé: | Engineering & Physical Science Research Council (E |
| Rok vydání: | 2016 |
| Předmět: |
Biochemistry & Molecular Biology
Otras Ciencias Biológicas glucan recognition neoglycolipids 11 Medical And Health Sciences 06 Biological Sciences CARBOHYDRATE MICROARRAY Ciencias Biológicas carbohydrate microarray NEOGLYCOLIPIDS Β1 2-GLUCAN C-type lectins β1 2-glucan C-TYPE LECTINS GLUCAN RECOGNITION CIENCIAS NATURALES Y EXACTAS |
| Popis: | The β1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic β1,2-glucan (CβG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems. Fil: Zhang, Hongtao. Imperial College London; Reino Unido. Jiangnan University; China Fil: Palma, Angelina S.. Imperial College London; Reino Unido. Universidade de Lisboa; Portugal Fil: Zhang, Yibing. Imperial College London; Reino Unido Fil: Childs, Robert A.. Imperial College London; Reino Unido Fil: Liu, Yan. Imperial College London; Reino Unido Fil: Mitchell, Daniel A.. University of Warwick; Reino Unido Fil: Guidolin, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Weigel, Wilfried. SCIENION AG; Alemania Fil: Mulloy, Barbara. Imperial College London; Reino Unido Fil: Ciocchini, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Feizi, Ten. Imperial College London; Reino Unido Fil: Chai, Wengang. Imperial College London; Reino Unido |
| Databáze: | OpenAIRE |
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