Dynamic neuroanatomy at subcellular resolution in the zebrafish
| Autor: | Faucherre, Adèle, Lopez-Schier, Hernán |
|---|---|
| Přispěvatelé: | Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2014, 1082, pp.187--95. ⟨10.1007/978-1-62703-655-9_13⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-62703-655-9_13⟩ |
| Popis: | International audience; Genetic means to visualize and manipulate neuronal circuits in the intact animal have revolutionized neurobiology. "Dynamic neuroanatomy" defines a range of approaches aimed at quantifying the architecture or subcellular organization of neurons over time during their development, regeneration, or degeneration. A general feature of these approaches is their reliance on the optical isolation of defined neurons in toto by genetically expressing markers in one or few cells. Here we use the afferent neurons of the lateral line as an example to describe a simple method for the dynamic neuroanatomical study of axon terminals in the zebrafish by laser-scanning confocal microscopy. |
| Databáze: | OpenAIRE |
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