Boronic acid-based autoligation of nucleic acids: influence of the nature of the 30-end ribonucleotidic strand

Autor: Barbeyron, Renaud, Wengel, Jesper, Vasseur, Jean-Jacques, Smietana, Michaël
Přispěvatelé: Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Nucleic Acid Center, Department of Physics, University of Southern Denmark (SDU)
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Chemical Monthly / Monatshefte für Chemie
Chemical Monthly / Monatshefte für Chemie, Springer Verlag, 2013, 144, pp.495-500. ⟨10.1007/s00706-012-0881-7⟩
ISSN: 0026-9247
1434-4475
DOI: 10.1007/s00706-012-0881-7⟩
Popis: International audience; The development of synthetic systems displaying dynamic and adaptive characteristics is a formidable challenge with wide applications from biotechnology to therapeutics. Recently, we described a dynamic and programmable nucleic acid-based system relying on the formation of reversible boronate internucleosidic linkages. The DNA- or RNA-templated system comprises a 50 -ended boronic acid probe connecting a 30 -ended ribonucleosidic oligonucleotide partner. To explore the dominant factors that control the reversible linkage, we synthesized a series of 30 -end modified ribonucleotidic strands. Evidence suggests that geometric and steric factors are key features for controlling the equilibria.
Databáze: OpenAIRE
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