Ocjena izloženosti kompleksu Penicillium glabrum u proizvodnji pluta s pomoću komplementarnih metoda
| Autor: | Carla Viegas, Raquel Sabino, Daniel Botelho, Mateus dos Santos, Anita Quintal Gomes |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Arhiv za higijenu rada i toksikologiju Volume 66 Issue 3 |
| ISSN: | 0004-1254 1848-6312 |
| Popis: | Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry. Hrast plutnjak druga je vrsta po učestalosti u portugalskim šumama, zbog čega je ta zemlja najveći izvoznik pluta na svijetu. Profesionalna je izloženost plijesnima Chrysonilia sitophila i Penicillium glabrum česta u proizvodnji pluta, a potonja vrsta povezana je s plućnom bolesti suberozom. Međutim, prilikom procjene izloženosti konvencionalnim se metodama često podcjenjuje prisutnost te vrste u radnom okolišu. Stoga je cilj ovog istraživanja bio provjeriti pretpostavku da polimerazna lančana reakcija (PCR) kao molekulska metoda dopunjuje nalaze konvencionalnih metoda i time daje bolji uvid u profesionalnu izloženost radnika u proizvodnji pluta. U tu smo svrhu istražili onečišćenje kompleksom P. glabrum u trima tvornicama pluta u okolici Lisabona oslanjajući se na konvencionalne i molekulske metode. PCR je otkrio prisutnost plijesni u šest uzoraka, u kojih konvencionalne metode nisu otkrile njihovu prisutnost. To potvrđuje našu pretpostavku da se primjenom komplementarnih metoda može steći bolji uvid i napraviti točnija procjena profesionalne izloženosti kompleksu P. glabrum u proizvodnji pluta. |
| Databáze: | OpenAIRE |
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