Reversal of ethinylestradiol induced cholestasis by Epomediol in rat. The role of liver plasma membrane fluidity
| Autor: | Miccio, M, Orzes, N, Lunazzi, Gc, Gazzin, Bruno, Corsi, R, Tiribelli, Claudio |
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| Přispěvatelé: | Miccio, M, Orzes, N, Lunazzi, Gc, Gazzin, Bruno, Corsi, R, Tiribelli, Claudio |
| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
Male
Cell Membrane/drug effects Animals Bicyclo Compounds Heterocyclic Bile/drug effects Cell Membrane/drug effects Cell Membrane/enzymology Cholagogues and Choleretics/pharmacology* Cholestasis/chemically induced* Cholestasis/prevention & control Ethinyl Estradiol/antagonists & inhibitors* Liver/drug effects* Liver/enzymology Male Membrane Fluidity/drug effects* Rats Rats Inbred Strains Sodium-Potassium-Exchanging ATPase/analysis Terpenes/pharmacology Heterocyclic Sodium-Potassium-Exchanging ATPase/analysis Inbred Strains Liver/enzymology Membrane Fluidity/drug effects Cholestasis/chemically induced Ethinyl Estradiol/antagonists & inhibitors Liver/drug effects Rats Bile/drug effects Cholagogues and Choleretics/pharmacology Cell Membrane/enzymology Animals Terpenes/pharmacology Bicyclo Compounds Cholestasis/prevention & control |
| Popis: | Epomediol (EPO) is a synthetic terpenoid compound shown to be active in increasing bile flow and some enzymatic activities of liver plasma membranes in the rat. The possible effect of EPO treatment in the ethinyl-estradiol (EE) induced cholestasis in the rat was investigated by measuring the hepatic transport of sulfobromophthalein (BSP) (plasma clearance and biliary secretion) and bile flow. Liver plasma membrane fluidity was also determined by the steady state fluorescence polarization (P) of diphenylhexatriene (DPH). EE administration (5 mg/kg s.c. for 5 days) was followed by a significant, comparable reduction (P less than 0.001) in BSP plasma clearance and biliary excretion and in bile flow. Intraperitoneal administration of EPO (100 mg/kg) to EE-treated rats restored both parameters of BSP transport, as well as bile flow, to control values. Liver plasma membrane fluidity was markedly (P less than 0.01) decreased by EE administration with a concomitant reduction (P less than 0.01) in Na+/K+-ATPase activity. EPO administration significantly increased membrane fluidity to values higher either to cholestatic (P less than 0.05) or control (P less than 0.05) animals. On the contrary, EPO did not influence Na+/K+-ATPase activity in either EE-treated or control animals. These data indicate that EPO fully reverses the impairments of BSP transport and bile flow induced by EE, possibly by reversing the decrease in liver plasma membrane fluidity induced by the synthetic estrogen. On the contrary, the EE-mediated decrease in Na+/K+-ATPase activity was not reversed by EPO. |
| Databáze: | OpenAIRE |
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