Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine
| Autor: | Pasandideh, R., Nassiri, M. R., Raouf Delgosha, M., Aslaminejad, A. A., mojtaba tahmoorespur, Zibaei, S., Doosti, M., Pasandideh, M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Archives of Razi Institute, Vol 71, Iss 1, Pp 15-19 (2016) Scopus-Elsevier |
| ISSN: | 2008-9872 0365-3439 |
| Popis: | Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed livestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs with FMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after the first vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes were upregulated. The expression of TNF-α and IL-10 genes significantly increased (P |
| Databáze: | OpenAIRE |
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