Osteopontin is up-regulated and associated with neutrophil and macrophage infiltration in glioblastoma

Autor: Atai, N.A., Bansal, M., Lo, C., Bosman, J., Tigchelaar, W., Bosch, K.S., Jonker, A., de Witt Hamer, P.C., Troost, D., McCulloch, C.A., Everts, V., van Noorden, C.J.F., Sodek, J.
Přispěvatelé: Orale Celbiologie (ORM, ACTA), Faculteit der Geneeskunde, Neurosurgery, CCA - Immuno-pathogenesis, MOVE Research Institute, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Cell Biology and Histology, Amsterdam Neuroscience, Pathology, Oral Cell Biology
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Atai, N A, Bansal, M, Lo, C, Bosman, J, Tigchelaar, W, Bosch, K S, Jonker, A, de Witt Hamer, P C, Troost, D, McCulloch, C A, Everts, V, van Noorden, C J F & Sodek, J 2011, ' Osteopontin is up-regulated and associated with neutrophil and macrophage infiltration in glioblastoma ', Immunology, vol. 132, no. 1, pp. 39-48 . https://doi.org/10.1111/j.1365-2567.2010.03335.x
Immunology, 132(1), 39-48. Wiley-Blackwell
ISSN: 0019-2805
DOI: 10.1111/j.1365-2567.2010.03335.x
Popis: P>Osteopontin (OPN) is a glycophosphoprotein with multiple intracellular and extracellular functions. In vitro, OPN enhances migration of mouse neutrophils and macrophages. In cancer, extracellular OPN facilitates migration of cancer cells via its RGD sequence. The present study was designed to investigate whether osteopontin is responsible for neutrophil and macrophage infiltration in human cancer and in particular in glioblastoma. We found that in vitro mouse neutrophil migration was RGD-dependent. In silico, we found that the OPN gene was one of the 5% most highly expressed genes in 20 out of 35 cancer microarray data sets in comparison with normal tissue in at least 30% of cancer patients. In some types of cancer, such as ovarian cancer, lung cancer and melanoma, the OPN gene was one of those with the highest expression levels in at least 90% of cancer patients. In glioblastoma, the most invasive type of brain tumours/glioma, but not in lower grades of glioma it was one of the 5% highest expressed genes in 90% of patients. In situ, we found increased protein levels of OPN in human glioblastoma versus normal human brain confirming in silico results. OPN protein expression was co-localized with neutrophils and macrophages. In conclusion, OPN in tumours not only induces migration of cancer cells but also of leucocytes
Databáze: OpenAIRE
Externí odkaz: