| Autor: |
Akatsuka, Maiko, Takayama, Yuriko, Muchevbe, Edwin, Ito, Kazunori, Watanabe, Kenta, Kuwae, Tomohiro, Minamoto, Toshifumi |
| Jazyk: |
japonština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
土木学会論文集B2(海岸工学). 76(2):I_943-I_948 |
| ISSN: |
1884-2399 |
| Popis: |
海草場の変化を捉えるモニタリング方法として環境DNAの活用を目指し，海草量の季節変化に伴うeDNA量の変化を把握することを目的に，水槽に生育するアマモを対象に15ヵ月の生育観察と環境DNA分析を実施した．また，実海域でのモニタリングに向けて，潮汐による流れの変化がある環境下で，季節変化の差に対して採水地点や同時刻に採水した差がどの程度であるのか調査した．15ヵ月のモニタリングでは，環境DNA量が夏に高い値を示し秋に低下する周期性を確認でき，海草が流出する時期に高くなる可能性が明らかになった．実海域調査では，同時刻に採水した１Lの分析で検出の有無が混在する結果となったが，採水量や分析量を増量し，阻害影響を低減することで定量下限を超えた値の検出が可能となり，実海域調査への適応が期待できる結果を得られた．The utilization of environmental DNA(eDNA) is being considered as a new method for monitoring seagrass beds. To understand the change in eDNA corresponding to seasonal variations in seagrass, the growth of Zostera marina inhabiting inside a water tank along with the amount of eDNA were investigated for fifteen months. Furthermore, investigations were performed under conditions where the flow changes due to the tide for the future monitoting in the sea. The investigations focused on the differernce of the eDNA collected at different sampling points and at the same time of a day on the seasonal changes. The fifteen month monitoring confirmed that the concentration of eDNA was high in the summer season which suggests that it is related to the increase in the amount of seagrass biomass and its release. As a result of the survey in the sea, the samples taken at the same time of a day showed a mixture of detection and non-detection. However, by increasing the amount of water sampled by 10 times, and the amount of analysis by 2.5times, and also by reducing the inhibition effect, detection was possible. Additionally, the results showed the possibility of applying the method in this study for future surveys in the sea. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
