Hypoxic pulmonary vasoconstriction, carotid body function and erythropoietin production in adult rats perinatally exposed to hyperoxia

Autor: Prieto-Lloret, Jesús, Ramirez, Maria, Olea, Elena, Castañeda, J., Yubero, Sara, Agapito, Teresa, Gómez-Niño, A., Rocher, Asunción, Rigual, Ricardo, Obeso, Ana, Pérez-Vizcaino, Francisco, González, Constancio
Přispěvatelé: Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III
Rok vydání: 2015
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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ISSN: 1469-7793
0022-3751
Popis: Adult mammalians possess three cell systems that are activated by acute bodily hypoxia: pulmonary artery smooth muscle cells (PASMC), carotid body chemoreceptor cells (CBCC) and erythropoietin (EPO)-producing cells. In rats, chronic perinatal hyperoxia causes permanent carotid body (CB) atrophy and functional alterations of surviving CBCC. There are no studies on PASMC or EPO-producing cells. Our aim is to define possible long-lasting functional changes in PASMC or EPO-producing cells (measured as EPO plasma levels) and, further, to analyse CBCC functional alterations. We used 3- to 4-month-old rats born and reared in a normal atmosphere or exposed to perinatal hyperoxia (55–60% O2 for the last 5–6 days of pregnancy and 4 weeks after birth). Perinatal hyperoxia causes an almost complete loss of hypoxic pulmonary vasoconstriction (HPV), which was correlated with lung oxidative status in early postnatal life and prevented by antioxidant supplementation in the diet. O2-sensitivity of K+ currents in the PASMC of hyperoxic animals is normal, indicating that their inhibition is not sufficient to trigger HPV. Perinatal hyperoxia also abrogated responses elicited by hypoxia on catecholamine and cAMP metabolism in the CB. An increase in EPO plasma levels elicited by hypoxia was identical in hyperoxic and control animals, implying a normal functioning of EPO-producing cells. The loss of HPV observed in adult rats and caused by perinatal hyperoxia, comparable to oxygen therapy in premature infants, might represent a previously unrecognized complication of such a medical intervention capable of aggravating medical conditions such as regional pneumonias, atelectases or general anaesthesia in adult life.
This work was supported by Grants BFU2012-37459 from the Ministry of Economy and Competitiveness (Spain) and Grant CIBER CB06/06/0050 from the Institute of Health Carlos III (Spain) to C. G. Also supported by Grants SAF2011-28150 to F.P-V and SAF2010-22066-C02-02 to AC from the Ministry of Economy and Competitiveness (Spain).
Databáze: OpenAIRE
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