Multidisciplinary characterization of interactions between oestrogen hormones and their receptors during sperm capacitation
| Autor: | Bosáková, Tereza |
|---|---|
| Přispěvatelé: | Komrsková, Kateřina, Smrček, Stanislav, Postlerová, Pavla |
| Jazyk: | čeština |
| Rok vydání: | 2022 |
| Předmět: |
tandemová hmotnostní spektrometrie
endokrinní disruptory tandem mass spectrometry endocrine disrupting chemicals high-performance liquid chromatography kinetic analysis vysokoúčinná kapalinová chromatografie sperm capacitation estrogenní hormony oestrogen hormones kapacitace spermií kinetická analýza |
| Popis: | This doctoral thesis was focused on the multidisciplinary characterizatrion of interactions between hormones and their receptors during sperm capacitation, which is the final step in sperm maturation in mammals and the key event to achieve egg fertilization. Three oestrogenic hormones (17β-estradiol (E2), 17α-estradiol (αE2), 17α-ethynylestradiol (EE2)), which are part of environmental pollution, were selected for the study. These hormones are called endocrine disruptors as they can cause disorders of the hormonal system in animals, thereby adversely affecting physiological processes, including reproduction. A new analytical HPLC-MS/MS method was developed for monitoring of concentration changes of oestrogens (E2, αE2 and EE2) during time-dependent capacitation of mouse and rat sperm in vitro. For individual oestrogens, the concentration dependences of free, unbound hormone on the capacitation time were measured for three concentrations of individual hormones (200, 20 and 2 μg/L). The obtained concentration data were converted into values of relative concentrations, which were subjected to kinetic analysis. Kinetic models were proposed for the interaction of individual hormones with sperm, which were described by a system of differential equations with optimization of rate constants and molar... |
| Databáze: | OpenAIRE |
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