Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30

Autor:	Ortiz, Gastón Ezequiel, Alberto, Edgardo Omar, Blasco, Martín
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	purl.org/becyt/ford/2 [https] Trichoderma reesei purl.org/becyt/ford/2.9 [https] Recombinant protein expres-sion ToxA promoter Weak promoter INGENIERÍAS Y TECNOLOGÍAS Biotecnología Industrial
Zdroj:	CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET
Popis:	In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30 Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial; Argentina
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::61895cb1ab1928047c8b6fcbd0bd1e8d http://www.tsijournals.com/abstract/functional-evaluation-of-toxa-promoter-in-trichoderma-reeseirutc30-2219.html Zobrazit plný text záznamu