Thermostable Cas9 nucleases with reduced off-target activity
| Autor: | van der Oost, J., van Kranenburg, R., Bosma, E.F., Mougiakos, I., Mohanraju, P. |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Popis: | ThermoCas9 is identified and characterized from the thermophilic bacterium Geobacillus thermodenitrificans T12. Experiments show how in vitro ThermoCas9 is active between 20 and 70 °C, has stringent PAM-preference at lower temperatures, tolerates fewer spacer-protospacer mismatches than SpCas9 and its activity at elevated temperatures depends on the sgRNA-structure. Described are ThermoCas9-based engineering tools for gene deletion and transcriptional silencing at 5 °C in Bacillus smithii and for gene deletion at 37 °C in Pseudomonas putida |
| Databáze: | OpenAIRE |
| Externí odkaz: |
https://research.wur.nl/en/publications/thermostable-cas9-nucleases-with-reduced-off-target-activity
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