| Autor: |
Beekman, B., El, B. van, Drijfhout, J.W., Ronday, H.K., TeKoppele, J.M. |
| Přispěvatelé: |
Gaubius Instituut TNO |
| Jazyk: |
angličtina |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
FEBS Letters, 3, 418, 305-309 |
| Popis: |
Stromelysin-1 (MMP-3) is an important member of the matrix metalloproteinase family. In joint-degrading diseases like arthritis, elevated levels of MMP-3 protein are detected in synovial fluid using immunological methods. However, these methods do not discriminate between active and inactive enzyme. In the present study, a specific stromelysin activity assay was developed using the selective fluorogenic substrate TNO003 (Dabcyl-Gaba-Arg-Pro-Lys-Pro-Val-Glu · Nva-Trp-Arg-Glu-(EDANS)-Ala-Lys-NH2, · = cleavage site). For its use in biological media, cleavage of TNO003 by enzymes other than stromelysin was effectively blocked by a proteinase inhibitor cocktail. Spiking of MMP-3 to synovial fluid resulted in an MMP-3 concentration-dependent linear increase in activity. The measured MMP-3 activity was not affected by the addition of MMP-13, even in a 5-fold excess over MMP-3. Synovial fluid from rheumatoid arthritis patients demonstrated 100-fold higher levels of active stromelysin than control synovial fluids. Chemicals/CAS: Biological Markers; Matrix Metalloproteinase 3, EC 3.4.24.17 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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