Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control
| Autor: | Lutrat, Célia, Burckbuchler, Myriam, Olmo, Roenick Proveti, Beugnon, Rémy, Fontaine, Albin, Akbari, Omar S, Argilés-Herrero, Rafael, Baldet, Thierry, Bouyer, Jérémy, Marois, Eric |
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| Přispěvatelé: | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Modèles Insectes de l'Immunité Innée (M3I), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), German Centre for Integrative Biodiversity Research (iDiv), Leipzig University, Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA), University of California [San Diego] (UC San Diego), University of California (UC), International Atomic Energy Agency [Vienna] (IAEA), ANR-11-EQPX-0022,I2MC,Insectarium pour l'Infectiologie Moléculaire et Cellulaire(2011), European Project: 682387,REVOLINC |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Male
CRISPR-Cas systems [SDV.BIO]Life Sciences [q-bio]/Biotechnology Genetically Modified Mosquito Vectors Insecticide Resistance Aedes aegypti Aedes Animal transgénique Animals Lutte anti-insecte Lâcher d'insectes stériles L10 - Génétique et amélioration des animaux Aedes albopictus Lutte biologique génétique animale Infectious Diseases Vecteur de maladie Larva Genetic engineering Female Entomology L72 - Organismes nuisibles des animaux |
| Zdroj: | Communications Biology Communications Biology, 2023, 6 (1), ⟨10.1038/s42003-023-05030-7⟩ Communications biology, vol 6, iss 1 |
| ISSN: | 2399-3642 |
| Popis: | International audience; Chemical control of disease vectoring mosquitoes Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is a valuable alternative but is limited by slow, error-prone, and wasteful sex-separation methods. Here, we present four Genetic Sexing Strains (two for each Aedes species) based on fluorescence markers linked to the m and M sex loci, allowing for the isolation of transgenic males. Furthermore, we demonstrate how combining these sexing strains enables the production of non-transgenic males. In a mass-rearing facility, 100,000 first instar male larvae could be sorted in under 1.5 h with an estimated 0.01-0.1% female contamination on a single machine. Cost-efficiency analyses revealed that using these strains could result in important savings while setting up and running a mass-rearing facility. Altogether, these Genetic Sexing Strains should enable a major upscaling in control programmes against these important vectors. |
| Databáze: | OpenAIRE |
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